Lotterhos Lab Protocols
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Molecular Protocols for Using the BioRAD qPCR Machine
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Autoclave (OGL)
Scope Camera MVI Nikon SMZ 1000
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Opentrons OT-2 Pipetting Robot
NUMSC - Shared Lab Space
Molecular Equipment
Take3 DNA Quantification
PicoGreen Assay
Magnetic Bead Wash
OMAX Microscope and Camera
TapeStation Library Check
Molecular Protocols for Using the BioRAD qPCR Machine
Pipetting
Pipette Decontamination
Seascape Sample Extractions App Use
Outdated Molecular Protocols
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Chett’s Cold Brew
Molecular Protocols
Types of Ethanol
Determining Barcodes and Indexes for Illumina Sequencing
DNA Extraction (Omega)
DNA Extraction (Qiagen)
Plate DNA Extraction (Qiagen)
DNA Extraction (Spartina)
Gel Electrophoresis
DNA Drying
Illumina Library Prep
Library Quantification
Qubit dsDNA Quantification
RNA Extraction
Solution Recipes
Changing Gonad Buffer
HCl Baths for Qiagen S-Blocks
Stable Isotope Protocol
Field and Sampling Protocols
Collecting Samples for DNA
Collecting Samples for RNA
Conductivity Loggers
Field Safety Protocol
Oyster Phenotypic Data Gathering
ImageJ & Mapping Oyster Shells
Lab Kayak Care
pH Loggers
Managing Seatables
Water Sampling
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Transferring Files md5
Accessing and Using Defiance Cluster
General Directory Organization
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Setting Alias or PATH variable
Pseudo-Parallelize Your Code on the Cluster
Setting Seeds for Reproducibility
Troubleshooting CellProfiler Installation
Basic Unix Commands
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Discovery
Connecting to the Discovery Cluster
Software and Containers on Discovery for Lotterhos Lab
Open OnDemand
Globus File Transfer
Lotterhos Lab vs. Personal Storage on Discovery and GitHub
Example Scripts
Workflow Parallelization Approaches
Optimizing
Parallelization with Array
Instructions for Discovery for Non-Northeastern Personnel
What are VS Code and PyCharm?
Bioinformagics
install iTerm terminal app for MacOS
Advanced SSH-ing
Installing Anaconda and Anaconda Environments
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How to color code your terminal
Outreach and Teaching
All About Oysters: Outreach Workshop
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TapeStation Library Check
Pipetting